AmScope B120C-DK-E1 Binocular LED Darkfield Microscope User Manual

소개

This manual provides instructions for the proper setup, operation, and maintenance of your AmScope B120C-DK-E1 Binocular LED Darkfield Microscope. This instrument is designed for educational, clinical, and laboratory applications, offering both brightfield and darkfield observation capabilities. Please read this manual thoroughly before using the microscope to ensure safe and effective operation.

안전 정보

• Always handle the microscope with care. Avoid sudden movements or impacts.
• 현미경이 넘어지지 않도록 안정적이고 평평한 표면에 놓으십시오.
• 청소 또는 유지 보수를 하기 전에 전원 코드를 뽑으십시오.
• 현미경을 직사광선, 고온 또는 고습에 노출시키지 마십시오.
• 지정된 전원 어댑터만 사용하세요.
• Keep the optical components clean. Avoid touching lenses with bare hands.
• Do not attempt to disassemble or repair the microscope yourself. Contact qualified service personnel.

구성 요소 및 기능

Familiarize yourself with the main components of your AmScope B120C-DK-E1 microscope:

그림 1: 위에view of the AmScope B120C-DK-E1 Binocular Compound Microscope, highlighting key features such as the 2-layer mechanical stage, 360-degree adjustable head, 40X-2500X magnification, coaxial coarse and fine focus, and LED sub-stag전자 조명.

그림 2: The Siedentopf binocular head, featuring 360-degree rotation and a 30-degree inclination for comfortable viewing and posture adjustment.

그림 3: 상세한 view of the 2-layer mechanical stage and the coaxial coarse and fine focus knobs, designed for precise specimen movement and focusing.

그림 4: The LED sub-stage lighting system, providing cool, energy-efficient, and daylight-balanced illumination for improved contrast and resolution.

주요 구성 요소:

• Siedentopf Binocular Head: Rotates 360 degrees with a 30-degree inclination for ergonomic view주고받음.
• 접안렌즈: Includes 10X and 25X widefield eyepieces for various magnification levels.
• 대물렌즈: Achromatic, color-coded objective lenses (e.g., 4X, 10X, 40X, 100X oil) mounted on a revolving nosepiece.
• 기계식 Stage: A 2-layer stage with low-position controls for precise X-Y movement of slides.
• Coaxial Focus Knobs: Separate coarse and fine focus adjustments for accurate focusing.
• LED 조명기: 서브tage LED light source with adjustable brightness.
• 콘덴서: Includes a brightfield condenser and a darkfield condenser for specialized observation.
• 1MP Digital Eyepiece Camera: For capturing images and videos, compatible with Windows, Mac, and Linux.

설정

1. 짐 풀기: 모든 구성품을 포장에서 조심스럽게 꺼내세요. 나중에 운반하거나 보관할 수 있도록 포장을 보관하세요.
2. 놓기: Place the microscope on a stable, vibration-free surface away from direct sunlight and heat sources.
3. 헤드 설치: Loosen the head retaining screw on the top of the microscope body. Gently insert the binocular head into the opening, ensuring it is fully seated. Tighten the retaining screw.
4. 접안렌즈 설치: Remove the protective caps from the eyepiece tubes. Insert the desired eyepieces (10X or 25X) into the tubes.
5. Objective Lens Installation: Rotate the revolving nosepiece to ensure there is no objective lens directly above the stage. Screw the objective lenses into the nosepiece in increasing order of magnification (e.g., 4X, 10X, 40X, 100X).
6. 전원 연결: 전원 코드를 현미경의 전원 입력 단자에 연결한 다음 적절한 콘센트에 꽂으십시오.
7. Digital Eyepiece Camera Installation: If using the digital eyepiece, remove one of the standard eyepieces and insert the 1MP digital eyepiece camera into the eyepiece tube. Connect the camera to your computer via USB.

작동 지침

1. Brightfield Observation

그림 5: Examples of magnification levels (10X, 100X, 400X, 1000X) achievable with the AmScope B120C-DK-E1, utilizing various objective lenses and eyepieces, including the additional 25X eyepieces for up to 2500X magnification.

1. 전원 켜기: Turn on the main power switch. Adjust the LED illuminator brightness using the dimmer control.
2. Stag전자 조정: 기계적 s를 낮추십시오tage using the coarse focus knob to create enough space to place a slide.
3. Slide Placement: Place a prepared specimen slide onto the mechanical stage, s로 고정tage clips. Use the X-Y stage controls to position the specimen over the light aperture.
4. 확대율 선택: Rotate the revolving nosepiece to select the lowest power objective lens (e.g., 4X). Ensure it clicks into place.
5. 초점:
   • While looking through the eyepieces, slowly raise the stage using the coarse focus knob until the specimen comes into rough focus.
   • Use the fine focus knob to achieve sharp focus.
   • Adjust the interpupillary distance (distance between eyepieces) and diopter adjustment on the eyepiece for comfortable viewing and to correct for differences in vision between your eyes.
6. 증분asing Magnification: Once focused at low power, rotate the nosepiece to a higher power objective (e.g., 10X, 40X). The microscope is parfocal, meaning only minor adjustments with the fine focus knob should be needed.
7. Using 100X Oil Immersion Objective:
   • After focusing with the 40X objective, rotate the nosepiece halfway between the 40X and 100X objectives.
   • Place a small drop of immersion oil directly onto the center of the specimen slide.
   • Rotate the 100X objective into the oil drop. The objective lens should make contact with the oil.
   • Use only the fine focus knob to bring the image into sharp focus. Do not use the coarse focus knob with the 100X objective.
   • After use, clean the 100X objective lens and the slide immediately with lens cleaning solution and lens paper.
8. 응축기 조정: Adjust the iris diaphragm on the condenser to control the amount of light and contrast. For higher magnifications, open the diaphragm more. For lower magnifications, close it slightly. The condenser can also be centered for optimal illumination.

2. Darkfield Observation

The AmScope B120C-DK-E1 includes a darkfield condenser for observing unstained, live specimens.

1. Condenser Exchange: Remove the brightfield condenser from its mount beneath the stage. Carefully insert the darkfield condenser into the mount. Ensure it is seated correctly.
2. 표본 준비: Darkfield observation works best with thin, unstained specimens.
3. 조명: Turn on the LED illuminator and set it to a high brightness level.
4. 초점: Use a low power objective (e.g., 10X or 20X, if available) first. Focus on the specimen as you would in brightfield. The specimen will appear brightly lit against a dark background.
5. Centering the Darkfield Condenser: Some darkfield condensers require centering. If your condenser has centering screws, adjust them until the darkfield effect is uniform across the field of view.
6. Higher Magnification: For higher magnifications, you may need to use a specialized darkfield objective or ensure your standard objectives are compatible. Always ensure sufficient immersion oil is used with 100X darkfield objectives.

3. Digital Eyepiece Camera Operation

The included 1MP digital eyepiece camera allows for image capture and live view컴퓨터에서 작업 중.

그림 6: The AmScope B120C-DK-E1 microscope connected to a laptop, demonstrating live digital viewing and image capture capabilities via the 1MP digital eyepiece camera.

1. 소프트웨어 설치: Install the professional microscopy software provided (or download the latest version from the AmScope website) onto your Windows, Mac, or Linux computer.
2. 카메라 연결: Insert the digital eyepiece camera into one of the microscope's eyepiece tubes and connect it to your computer via the USB cable.
3. 소프트웨어 실행: Open the installed microscopy software. The software should detect the camera automatically.
4. 살다 View: The software will display a live feed from the microscope. Adjust microscope focus and illumination for the best image.
5. 이미지/비디오 캡처: Use the software's functions to capture still images or record videos of your observations. The software also provides tools for image processing and measurement.

유지

• 렌즈 세척: Use a soft brush to remove dust from lens surfaces. For smudges or oil, use a small amount of lens cleaning solution on lens paper. Wipe gently in a circular motion. Never use abrasive cloths or harsh chemicals.
• 세척 본체: 현미경 본체를 부드러운 천으로 닦으십시오.amp cloth. Avoid getting moisture into electrical components.
• 저장: 사용하지 않을 때는 먼지와 이물질로부터 현미경을 보호하기 위해 먼지 덮개를 씌우십시오. 서늘하고 건조한 곳에 보관하십시오.
• 침수 오일: Always clean immersion oil from the 100X objective and slides immediately after use. Dried oil can be difficult to remove and can damage the lens.

문제 해결

명세서

보증 및 지원

The AmScope B120C-DK-E1 microscope comes with a 5년 제조업체 보증. For warranty claims, technical support, or service inquiries, please contact AmScope customer service directly.

For additional resources, software downloads, and frequently asked questions, please visit the official AmScope web대지:

www.amscope.com